Ukugcina Ukunciphisa: Umlolongwane Wesikwekwe Esikhathini Sokuhlola

Ukufunda Ukunciphisa Kwe-Pipette Ngenkinga Yayo

Ukubonisa nge-Pipette Calibration ngixaki?

Ukubonisa nge-pipette calibration uyisingiseko olungempumelelo olunikezela ukuba amapipetti angqinisekise ukuze zibuyele umnotho omkhulu kwiindaweni zokulabula. Lolu hlobo lokusebenza luhluke kungcono ngoba lingenza ukuba izilimo zingezwe zizihambisane nokuphucumana. Ngiyeyiphi indlela yokubuyela, isenzo sesipipetti soshintsha ngaphandle kokuthiwe yindlela ezidinga ukukhathalwa kunye nezi-zilevela ezithatha imigomo, okungakho kube ngenxa yokushesha okanye ukusandiswa, okungayazi ukuba ayiphindaphindi. Ngexa lokhu lokuhlola lokugcina, uzobuya ngezinye izilimo ezifanele kuzo, uzokwenzela ukubheka phakathi komsebenzi wakho kunye nokubonisa, okunguqukethwe njengelokhu lokuhlola.

Yingaba Ukubonisa Ngokwengezi Kuningi Libungaza Ukuphucumana Kwesilabulo

Ukubuyisela ngokwesiphumo sifuna isithombe esincane kuhlukelela ukuhlukelela kwesilabulatho ngokufana nezinye izinto ezinethuthukiswa zokuphuhlwa ezikwenzeka ukuba amandla. Izibalo zithi ukuba okungakwazi ukusebenzisa i-pipet ngokucacile kungaba ngenxa yokuba yiziphi zihlabeleka, ukusetyenziswa kokubuyiselwa kuzodluliselwa ukuba zingenelelwe. Ngokushesha, uphawu olunye ku-70% lokungenziwa amandla labasebenzi ababangako kwi-labulatho kungabonisiwe ngaphandle kokuba kusebenzise i-pipet ngokubona. Lento ithemba lokuthi ukuba kuhlukelela ukubuyisela ngokwesiphumo. Ukuthi, amanani apho akhethiwe amaphuzu angazama ukusetshenziswa kwi-labulatho ayisho amalawulo ahlalekayo okudinga ukubuyisela i-pipet, ukusho ukuthi amalawulo akhethwe ngamanye amagama. Amalawulo amaningi akhethiwe ukuthi amandla alahlalekayo akhethwe ukuthi amandla alahlalekayo akhethwe ukuthi amandla alahlalekayo akhethwe.

Amathupha Akhethwe Ukuze Usenze Labulatho

I-Pipet Yesichannel Esidingi vs. I-Pipet Yesichannel Eziningi

Izinyathelo zokugcina amagama eliphakathi, ezinikela ngokwesicwangciso kwaye ngenkinga, bengamandla okukhangelwa ngaphandle kwezilabulali. Izihlabelela nokuthi izisebenzisi namanye amagama athisha amalungele angahlala ngokubekisiso phakathi kwezindlela ezinhloko, ukuthi abangene ngezinto ezingazange zisethwe ngendlela enkulu. Ngokugcineneyo, izinyathelo zenye iindlela zizosetshenziswa ukuthi ukuhlalela kakhulu lokusebenza ngokungasebenzi ngasinye esikhundleni sokuthola amachaneni, ngokuphelele kakhulu kunoma umsebenzi wemithwalo wechaneyi. Lolu hlobo lomsebenzi ulayishayo ngokukhangelwa kakhulu ngaphandle kwezilabulali ezinethuthuko, njenge-umthwalo womphakathi wokuthola imibophelelo yesintsha okanye yombuzo wesintfo somolekulo, apho kukho ukunquma lokusebenza amasampula ngasinye ngakwazi ukubonisa uphawuli. Ukusetha kakhulu phakathi kwesi nyathelo esingaphakathi kanye nenye iindlela ziyidinga ngokwesigaba sokuqukethwe sesiqhaza kunye nezinyathelo zenye iindlela zizofuneka ngokudinga ngokwesigaba sokuthola isiqu samasampula, ngaphandle kokuba zisebenzise ukuthola amasampula ngasinye.

Ukukhetha Ukuphetha Olunomvume kakhulu Lwesayithi Sakho

Ukukhetha ukuphetha olukhululekile liphindaphinda ukubona amandla ngokushesha, i-volume range, umthetho wemilongo akho, kunye nomphathiso wesicelo sokuqala. Ngokugcina, izayithi ezazisetyenziswa amalendawo ahlukileyo angakho amaphetha enhle ngeenhlelo zokuthumela. Okuhle kuqala, ukukhetha phambi kokuba manual okanye automatic pipettes kungabonisa isenzo sokugcina kwesayithi; izipipetha ezinamandla zikhona kuzimisele ekusetyenziswa lokuhlukanisa uphahla nokuphindelela. Ngenxa yobizo labizini, amameko eziyindlela nakuvamile njengama-Eppendorf kunye ne-Gilson, bayizange babuyeke kwezinye izigama zezameko ezifaneleleyo. Ngokufaka ukukhetha ukuphetha ngezinye izicwangciso kunye nemibuzo yobisi, izayithi zakho zingakwazi ukusebenzisa amaxwebhu abaningi kunye nokuhlinza imigomo yokuthuthukisa kunye nokuphahla.

Amathuluzi Amahluhlu kunye noMisebise Ezincane Zokuthatha Isilondolozi

Izinhlobo Zokuthatha Izilondolozi Zokuphetha Ezidingekayo

Ukusure yindlela elikhulu lokuhlola amapipeti, amathuluzi angaphambili ayishayamile. Yeyokuqala, iimvume zokuhlola zikhoyo kuhlu lapho zikhona ngokugcino ukukhetha amaligiqi ngempawu; kufuneka bazifise umnotho wokusondela kunye nomncane weemikrogramu. Yesibini, iimitholo zamahlola, okanye amanani e-ASTM class 1 noma E2 class, zisetshenziswa ukuvavanya umnotho wemvume yokuhlola. Yesithathu, amastandawd sihlolwayo apho akhethwe ukuvavanya ukwakhiwo kwamapipeti ngokubonisa izicatulo ezithethwe. Lephenduleti lezinzalo zifanele ukuvavanya ukuthi amapipeti azikhono kunye nokungakhulu kuhlola phakathi komphawu wokuhlola. Izithomo nemibhalo Mettler Toledo kunye neSartorius zikhona ngokupheleleyo ngoba babekho imininingwane yesiphumo sokuhlola kunye nokuncedisi okungabhedele.

Ukubuyisela Indawo Yomoya Wokuhlola Ukusonywa Kakhulu

Umsebenzi wokuhlinza ngokubalulekile kuyinhlangano elungileyo lokuhlola amanqaku enhlaweni yepipethi. Izinsuku ezidingekayo, njengokuthi ngakho i-ISO 8655, kunye nesiphumo esisenzelayo sifuneka zibhekisele. Ngakwesoku, ukususa ukukhombisa okanye ukususa izindlovu zingabheke ukuba zikhuphe umnotho wesimanje. Amaphathi akukho abakhulisene nokuthi babaleke amabhedlela ukuba babuyekeze uphawu lwesimanje. Kufanele amaphathi abasebenzise amabhedlela amaphrofetikali ukuba babheke ukuhambisana nesiphumo sokugcina. Izimo eziphumaphansi zifuneka zihlale ngokulungileyo ukuba zikhuse laba lokuhlola, uzokusure ka khona nokuhlola kahle nokuthi kuhlobene neentsha yokusebenza.

Umnotho Womphathiso WePipette Ngamavubi

Ukugcina Indawo Yomsebenzi Ngenqanawe

Ukugcina isikhomi sesithathu sikhona kuhlu lwezinto ezidingekayo lokusebenza ukuthi uqaphe phipha ngokubonakaleyo. Qala ngekhetho lokhu ukuthi usheshi lwesikhomi sifanele siyikhotho nokuthi asikho amagunya, okungafanelela ukuphatha. Kufanele ukwazi ukususa ikhethelo yezinto ezindilinga njengamashumi amabili, amandla amaphetheleyo, kunye neziphi zemiphiphethi yezikhomi ezilondoloziwe ngokuthi zihlabeleke ekuziphatheni. Ukusukela kuhlu lwezinto zikhona ngokuhlola ukuthi izinkambu zonke zikhona ngokuqinile, futhi uzokusiza ukukhetha ngokubonakaleyo. Isikhomi esisheshekile singakhotholeni lokusebenza kodwa sikuphumelela ukusuka kwezinhlobo zezimpawu.

Ukusebenzisa Imibala Yomdaka

Ukukhishwa kwezinto ezimbili ngokushesha yisimo sokuqinisekisa, ufakela ukukhangeza umkhuhlane obulawuliwe ngepipeti ukuze uphele lokunqume. Ukufaka lokhu, shintshisa umkhuhlane ngempumulo emfihlweni wekhontere enalokho lomoya oluhlupheke futhi rekorda ubuncinci. Lokhu kungena ukuba uhlola ubuncinci bokwentsha nobungako bezilungu zokubizwa, uzame ukubona ukuba indawo yokugcina yezilungu zikhethe iziqembu zezilungu ezibizwayo, njengamanzi, okungabangela amandla. Ngokushesha, amandla amahhale esikhetseni kuzobanjiswa ngokwesikhatsi sokuphela kwesiko sokubizwa, lokhu kudinga ukusetha kulesiko. Le siko somncane sokuphinde ukuba uhlola amandla apho apha, ukuze uphele lokunqume ngokupheleleyo ngaphandle kwezilungu nezinto eziphambene.

Ukubala nokuhlola Amandla

Kwisayizi, ukubuyilelwa kuhlula ukuba ngaphambili lokuphawulekileyo kunini ukuba liphilile ngale nani lokulandelwa, xa kunye nokuba ukuqinisekiswa kuhle kuhlala kusenokubonisa ukuphawulekiswa. Amametrici amahluke apha ahlukene nakwesiboniselo sokuthi zikhona izilungiselelo ezidingekayo. Ukukhetha kwabo kufuneka kuzosetshenziswa ngamamethodi yama-statistiki njengokubuya ku-Mean Absolute Error yokubuyilelwa kunye ne-Coefficient of Variation yokubonisa. Izicwangciso eziqinisekisekileyo zihlinza umpho wemibono yezilandelweni ezidingekayo ngokusho ukuba kuna imisindo yesisisekelo esizokususa izilandelweni. Ukubuya kwezinyathelo zakho zingabiza izimprofi zokugcina kunye nokuhlola kwisekhombisi esithathelakalo sasethemba.

Ukwenyusha Izinhlangano Eziningi

Ngaphandle kokuthi ukuhambisana kwiqinisekiso lwenqanawe zikhona, kungaba ngenxa yokuba kufanele ukusetha amandla ekugcwaleni okanye ukusetha i-setting ezithile zokusebenzela umoya. Kukho lokhu oluhle ukubonisa zonke izinhlobo ezazihamba nokuhlala ngendlela apho zazicabanga, ngoba izindlela ezilandelayo zisemva njengamaphrojekthi esebenza phakathi komkhawulo wokuhlola ephakathini. Ukugcwalisa i-integrity yokuqinisekiswa ngemuva kokusetshenziswa kufuneka asebenze ukuthi ameasurement esiqedwe asifane kakhulu nokuthi sibonisa isenzo sasendlela. Ukutesta ngemuva kokusetshenziswa kufanele ukumakelela ukuthi izinhlobo zizobuyelela uphawu oluphephile oluphumelele, lokhu kunikeza indlela eliphakamisiwe nokuthi akukho imposibhulo.

Ukulondoloza Ukuphumula: Ukulalela Izinkinga Ezimbili

Ukubophelela nokuthola Ukuphuma Kwicalibration

Ukuphopha kwezicalibration kukhiqiza ngokushesha ukupheleleka kweziqhuthu zokuhlola ngemvume yepipethi ngalokuvukile, kungenziwayo ngeentsingiselo ezincedisiyo nokuchange kweendaweni. Ukucacisa ukuphopha kwezicalibration, kuhluke ukubonelela ukusetyenziswa kwepipethi phakathi komunye westandardi okwenzeka nokufundisa amanani angaphambili. Izindlela zokugcina uphopha luchasene ngekuhlola kabusha eliphambili lokulandelayo nokuhlola labantu ababehlala kwindawo yokugcino labo. Ukubonelela ngokuzidingekile kuhluke ukungapheleleyo izilimo zokuthi. Le ncamo ucaciselela amanani amahlukeni nokuphazamisa, uzogcina uhlobo lwesayensi.

Amapheramusi Apho Amanje Ngokulandelayo Kuhlonela Epipethi

Ukugcina ukuqinisekiswa kwe-pipette ngomoya omkhulu ungenziwa isilondolozi esidingayo ukuguquka lokuphucula nokufinyelela. Amasilondolozi asingiseko luhlola ukuthi zihlabelele amanani apho akukho umphathano, uphinde ukugcina kahle kwise-case yokuhlala, kunye nolusapho olunemva kwezikhetho ezimelingayo ze-pipette. Ngizohlola lokungakho kuhluke ukubusa abasebenzisi kuzama izinto eziphathelene ne-pipetting, uzame ukususa okungavuthu ngokulandelayo phambi komoya wokuwamiswa. Ngiyaziya lokungapheleleyo, hayi amagcinelo amaningi:

• Amagcinelo :
  • Bheka phakathi kanye nokugcina i-pipette ngokujikeleza.
  • Hlala kwise-milenvu engasihlobo somfula.
  • Sebenzisa amathixo angahlala kwise-manufacturer.
• Amagcinane :
  • Ukusebenzisa amathuthi okhishiniwe noma ezidingekileyo kufuneka uzihlangele.
  • Usure pipetela ngokushayo emuva lokugcina lomhla ngamabili.
  • Phinda ukusetyenziswa kwiintemperethi ezikhulu.

Ngokufola kwezindlela ezibhekene nokuhlala, uhlobo olupheleleyo kunye nephandleni sokuqinisekisa le pipetela kungahlanganisiwe ngempumelelo kwisekhono esilabheni.